RESUMO
OBJECTIVE: To study the coumarin glucosides of Angelica dahurica. METHODS: Fresh roots and rhizomes of Angelica dahurica were extracted with ethanol at room temperature. Repeated column chromatography and preparative HPLC were used to isolate and purify the compounds. Their structures were elucidated on the basis of chemical evidence and spectral analysis. RESULTS: Twenty-six coumarin glucosides were isolated from Angelica dahurica, while here we reported 7 of them: sec.-O-beta-D-Galactopyranosyl-(R)-byakangelicin (I);8-O-beta-D-Galactopyranosyl-xanthotoxol (II); 7-O-beta-D-Apiofuranosyl-(1-->6)-beta-D-Glucopyranosyl-peucedanol (III); (R)-peucedanol-7-O-beta-D-Glucopyranoside (IV); sec.-O-beta-D-Glucopyranosyl-(R)-Oxypeucedaninhydrate (V); 7-O-beta-D-Galactopyranosyl-Sco-poletin( VI); Aesculin (VII). CONCLUSION: Compound V is a new compound, Compound VII is isolated from Umbellifera for the first time, compounds III, IV are isolated from Angelica for the first time,while compound I, II and VI are isolated from this plant for the first time.
Assuntos
Angelica/química , Cumarínicos/química , Glucosídeos/química , Raízes de Plantas/química , China , Cumarínicos/isolamento & purificação , Etanol/química , Furocumarinas/química , Furocumarinas/isolamento & purificação , Glucosídeos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Rizoma/químicaRESUMO
OBJECTIVE: To study the chemical constituents of Salicornia europaea. METHODS: The chemical constituents were isolated and purified by means of several chromatographic techniques and their structures were elucidated by spectroscopic methods. RESULTS: Five compounds were isolated and identified as syringaresinol 4-O-beta-D-glucopyranoside(1), icariside B2(2), erythro-1-(4-O-beta-D-glucopyranosyl-3,5-dimethyoxyphenyl)-2-syringaresinoxyl-propane-1,3-diol(3), longifloroside B(4), 7-O-beta-D-glucopyranosyl-6-methoxychromone(5). CONCLUSION: Compounds 1-4 are obtained from this genus for the first time.
Assuntos
Chenopodiaceae/química , Flavonas/isolamento & purificação , Glucosídeos/isolamento & purificação , Lignanas/isolamento & purificação , Plantas Medicinais/química , Flavonas/química , Glucosídeos/química , Lignanas/química , Espectroscopia de Ressonância Magnética , Estrutura MolecularRESUMO
OBJECTIVE: To study the chemical constituents from the bulbs of Zephyranthes candida. METHODS: The chemical constituents were separated and purified by means of several chromatographies and their structures were elucidated by spectroscopic methods. RESULTS: Six compounds were isolated and identified as trisphaeridine (I), p-hydroxybenzaldehyde (II), sucrose (III), (-)-amarbellisine (IV), lycorine (V), (+)-haemanthamine (VI). CONCLUSION: Compounds I - IV are obtained from this genus for the first time.
Assuntos
Benzaldeídos/isolamento & purificação , Dioxóis/isolamento & purificação , Liliaceae/química , Fenantridinas/isolamento & purificação , Alcaloides de Amaryllidaceae/química , Alcaloides de Amaryllidaceae/isolamento & purificação , Benzaldeídos/química , Dioxóis/química , Etanol/química , Espectroscopia de Ressonância Magnética , Fenantridinas/química , Raízes de Plantas/química , Sacarose/química , Sacarose/isolamento & purificaçãoRESUMO
OBJECTIVE: To study the chemical constituents of the aerial part of Bupleurum chinense. METHODS: Dried aerial parts of Bupleurum chinense were extracted with EtOH, the chemical constituents in the extract were isolated and their structures were identified by physical and chemical properties and spectral analysis. RESULTS: Five compounds were obtained and identified as 1-O-caffeoylglycerol (1), esculetin (2), scopoletin (3), kaempferol-3-O-beta-D-rutinoside (4) and alpha-spinastero (5). CONCLUSION: Compounds 1-3 are obtained from Bupleurum chinense for the first time.
Assuntos
Bupleurum/química , Lactonas/isolamento & purificação , Plantas Medicinais/química , Umbeliferonas/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Flavonoides/química , Flavonoides/isolamento & purificação , Glucosídeos/química , Glucosídeos/isolamento & purificação , Lactonas/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Folhas de Planta/química , Caules de Planta/química , Escopoletina/química , Escopoletina/isolamento & purificação , Umbeliferonas/químicaRESUMO
Two novel ceramides, Candidamide A (1) with a phytosphingolipid structure, and Candidamide B (2) with a tertiary amide structure, together with 12 known compounds (3-14) have been isolated from the bulbs of Zephyranthes candida, The structures of 1 and 2 have been elucidated to be 1,3,5,6-tetrahydroxy-2-(2'-hydroxytetracosanoyl amino)-8-(E)-octadecadiene (1) and (2S,3S,4R,8E,2'R)-2-[N-(2'-hydroxyoctadecanoyl)-N-(1'',2''-dihydroxyethyl)-amino]-8-hexacosene-1,3,4-triol (2) on the basis of spectroscopic evidence including IR, MS, NMR ((1)H-NMR, (13)C-NMR, DEPT, (1)H-(1)H COSY, HSQC, HMBC). The known compounds were identified as (2S)-3',7-dihydroxy-4'-methoxyflavan (3), (2S)-4'-hydroxy-7-methoxyflavan (4), (2S)-4',7-dihydroxyflavan (5), 7-hydroxy-3', 4'-methylenedioxyflavan (6), ambrettolide (7), beta-sitostero1 (8), beta-daucosterin (9), rutin (10), pancratistatin (11), lycorine (12), haemanthidine (13), and haemanthamine (14). In the antimicrobial assay, candidamide A (1) and candidamide B (2) displayed moderate activities against bacteria Staphylococcus aureus and Escherichia coli, and fungi Aspergillus niger, Candida albicans and Trichophyton rubrum.
Assuntos
Ceramidas/química , Liliaceae/química , Ceramidas/farmacologia , Espectroscopia de Ressonância Magnética , Viabilidade Microbiana/efeitos dos fármacos , Estrutura Molecular , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
OBJECTIVE: To study the chemical constituents of flower buds of Lonicera macranthoides. METHODS: The 90% EtOH extract of Lonicera macranthoides. was successively partitioned with petroleum ether and ethyl acetete. RESULTS: Repeated column chromatography of the ethyl acetete fraction afforded the following compounds (1-9): ginnol (1), triacontanol (2), ursolic acid (3), beta-sitosterol (4), triacontane (5), palmitic acid (6), beta-daucosterol (7), 3-decyl-3-octyldocosan-1-ol (8), 3-dodecyl-3-nonyldocosan-1-ol (9). All compounds except 4 are isolated from this plant for the first time while compounds 2, 3, 5, 8 and 9 are their first time been isolated from genus Lonicera.
Assuntos
Álcoois Graxos/isolamento & purificação , Lonicera/química , Parafina/isolamento & purificação , Plantas Medicinais/química , Triterpenos/isolamento & purificação , Álcoois Graxos/química , Flores/química , Espectroscopia de Ressonância Magnética , Ácido Palmítico/química , Ácido Palmítico/isolamento & purificação , Parafina/química , Sitosteroides/química , Sitosteroides/isolamento & purificação , Triterpenos/química , Ácido UrsólicoRESUMO
OBJECTIVE: To study the chemical constituents of the roots of Angelica pubescens. METHODS: Fresh roots of Angelica pubescens were extracted with EtOH,the chemical constituents in the extract were isolated by column chromatography as macroporous absorption resin,and ODS etc. Their structures were confirmed by spectral analysis (NMR, MS). RESULTS: Seven compounds were isolated and their structures were identified as nodakenetin (I), umbelliferone (II), columbianetin (III), beta-D-glucosyl-columbianetin (IV), columbianin (V), tert. -O-beta-D- glucopyranosyl -(R)-by-akangelicin (VI) and sec. -O-beta-D- glucopyranosyl-(R) -byakangelicin (VII). CONCLUSION: Compound IV, VI and VII are isolated from Angelica pubescens for the first time.
Assuntos
Angelica/química , Cumarínicos/isolamento & purificação , Furocumarinas/isolamento & purificação , Plantas Medicinais/química , Umbeliferonas/isolamento & purificação , Aconitina/análogos & derivados , Aconitina/química , Aconitina/isolamento & purificação , Cumarínicos/química , Furocumarinas/química , Glucosídeos/química , Glucosídeos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Raízes de Plantas/química , Umbeliferonas/químicaRESUMO
OBJECTIVE: To study on the chemical constituents of Zephyranthes candida. METHODS: Compounds were isolated and repeatedly purified by chromatographic techniques on silica gel column. Their structures were elucidated by chemical and spectral methods. RESULTS: Eight compounds were isolated from the whole plant of Zephyranthes candida, and identified as (2S)-3',7-dihydroxy-4'-methoxyflavan (I), beta-sitosterol (II), stigmasta-5,22-dien-3beta-ol (III), stigmasterol (IV), beta-daucosterin (V), Butylisobutyl phthalat (VI), Heptacosane (VII) and Ambrettolide (VIII). CONCLUSION: I-XIII are all isolated from this plant for the first time.
Assuntos
Flavonoides/isolamento & purificação , Liliaceae/química , Plantas Medicinais/química , Estigmasterol/isolamento & purificação , Ácidos Dicarboxílicos/química , Ácidos Dicarboxílicos/isolamento & purificação , Flavonoides/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Sitosteroides/química , Sitosteroides/isolamento & purificação , Estigmasterol/químicaRESUMO
OBJECTIVE: To study on the chemical constituents of root of Cynanchum auriculatum Royle ex Wight. METHODS: The constituents were isolated and purified by various chromatographic methods, and the spectroscopic methods (MS, NMR) were used to identify their sturctures. RESULTS: Seven compounds were obtained and identified as: taraxasterol acetate (I), cynanchone A (II), succinic acid (III), betulinic acid (IV), kidjoranin (V), wilfoside CIN (VI), wilfoside KIN (VII). CONCLUSION: Compounds I-IV are isolated from this plant for the first time.